Whole Genome Amplification:Article Title: Hepato-cardiac interorgan communication controls cardiac hypertrophy via combined endocrine-autocrine FGF21 signaling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF21 Novateinbio Cat#ASA-B0701; RRID:AB_3676235 pAMPKThr172 Cell Signaling Cat#2535; RRID:AB_331250 AMPK Cell Signaling Cat#2532; RRID:AB_330331 pERK1/2 Cell Signaling Cat#9101; RRID:AB_331646 ERK Cell Signaling Cat#4695; RRID:AB_390779 PPARα Santa Cruz Biotech Cat# Sc-398394; RRID:AB_2885073 Sirtuin 1 Cell Signaling Cat# 8469; RRID:AB_10999470 pAKT Ser473 Cell Signaling Cat# 9271; RRID:AB_329825 AKT Cell Signaling Cat# 9272; RRID:AB_329827 OXTR Proteintech Cat# 23045-1-AP; RRID:AB_2827425 β-Actin Santa Cruz Biotech Cat# SC-47778; RRID:AB_626632 Goat anti-Mouse IRDyeR 800CW LI-COR Cat#925–32210; RRID:AB_2687825 Goat anti-Rabbit IRDyeR 680RD LI-COR Cat#926–68071; RRID:AB_10956166 Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) Vector Labs Cat# FL-1021; N/A Bacterial and virus strains MyoAAV91A-cTnT-FGF21 Generated for this study by the Molkentin Laboratory N/A Biological samples Myocardial tissue from humans National Disease Research Interchange (NDRI) N/A Chemicals, peptides, and recombinant proteins DAPI Vectors Labs Cat#H-1500 Claycomb basal medium Sigma Cat # 51800C-500 Norepinephrine Sigma Cat#A0937 L-Glutamine Sigma Cat# TMS-002-C penicillin/streptomycin EMD Millipore Cat #TMS-AB2-C Lipofectamine 2000 Thermofischer Cat #11668019 PEI MAX MW 40K Polysciences Cat#24765-1; CAS#:49553-93-7 TRIzol lysis reagent ThermoFisher Cat#15596026 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 SYBR Select Master Mix Applied Biosystems Cat#4472903 BioRad FGF21 primer assay BioRad Cat#10025636 RIPA buffer RPI/VWR Cat#R26200–250.0 Pierce Protease and phosphotase inhibitor tablets Fisher Scientific Cat#PIA32961 PVDF membranes Fisher Scientific Cat#IPFL00010 Heparin Fisher Scientific Cat#NC2114549 Collagenase type II Worthington Biochemical Cat#LS004177 rh-FGF21 R&D Systems Cat #2539-FG-025/CF rm-FGF21 R&D Systems Cat #8409-FG-025/CF oxytocin peptide science Cat# 31-120-PS; CAS#50-56-6 (Continued on next page) e1 Cell Reports Medicine 6, 102125, June 17, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FGF21 Human ELISA Kit BioVendors RD191108200R Quantikine ELISA Mouse/Rat FGF-21 R&D SYSTEMS MF2100 Milliplex Inflammatory Cytokine Multiplex Millipore MCYTOMAG-70K-05 Deposited data Raw data files for RNA-seq Generated for this study; CCHMC Genomics Sequencing Facility NCBI Bioproject accession number: PRJNA1106030; Series GSE266368, GSE266957, GSE267618 Raw data files for Metabolomic Generated for this study; CCHMC Translational Metabolomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003743 Raw data files for Lipidomic Generated for this study; CCHMC Translational Lipidomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003851 Experimental models: Cell lines Human ventricular cardiomyocyte cell line (AC16) ATCC Cat#CRL-3568 Mouse cardiomyocyte cell line (HL-1) MiliporeSigma Cat#SCC065 AAVpro 293T cells Takara Bio Cat#632273 Experimental models: Organisms/strains HEP-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A CM-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A C57BL/6 Mice Jackson Laboratory Cat#000664 Oligonucleotides qRT-PCR primers Designed by the Drosatos Lab; obtained from Fisher Scientific Table S12 ASO-FGF21 Creative Biolabs Table S10 Recombinant DNA pAV-cTNT plasmid Obtained from Vigene Biosciences by the Molkentin Laboratory Cat#PM10013 Software and algorithms ImageJ ImageJ Software https://imagej.nih.gov/ij/ Graphpad Prism 10.2.1.395 Graphpad software https://www.graphpad.com/ TopSpin 3.6 Bruker BioSpin (NMR data collection/processing) https://www.bruker.com/en/products-andsolutions/mr/nmr-software/topspin.html Chenomx NMR Suite 8.1 Chenomx inc. (NMR data processing/ metabolites quantification) https://www.chenomx.com/ Vevo LAB 5.7.1 Fujifilm VisualSonic Vevo LAB (Echocardiography) https://www.visualsonics.com/ product/software/vevo-lab Progenesis QI Analysis Software Waters (LC-MS Data Analysis) https://www.waters.com/nextgen/us/en/ products/informatics-and-software/ mass-spectrometry-software/ progenesis-qi-software/progenesis-qi.html?
Virus:Article Title: Hepato-cardiac interorgan communication controls cardiac hypertrophy via combined endocrine-autocrine FGF21 signaling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF21 Novateinbio Cat#ASA-B0701; RRID:AB_3676235 pAMPKThr172 Cell Signaling Cat#2535; RRID:AB_331250 AMPK Cell Signaling Cat#2532; RRID:AB_330331 pERK1/2 Cell Signaling Cat#9101; RRID:AB_331646 ERK Cell Signaling Cat#4695; RRID:AB_390779 PPARα Santa Cruz Biotech Cat# Sc-398394; RRID:AB_2885073 Sirtuin 1 Cell Signaling Cat# 8469; RRID:AB_10999470 pAKT Ser473 Cell Signaling Cat# 9271; RRID:AB_329825 AKT Cell Signaling Cat# 9272; RRID:AB_329827 OXTR Proteintech Cat# 23045-1-AP; RRID:AB_2827425 β-Actin Santa Cruz Biotech Cat# SC-47778; RRID:AB_626632 Goat anti-Mouse IRDyeR 800CW LI-COR Cat#925–32210; RRID:AB_2687825 Goat anti-Rabbit IRDyeR 680RD LI-COR Cat#926–68071; RRID:AB_10956166 Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) Vector Labs Cat# FL-1021; N/A Bacterial and virus strains MyoAAV91A-cTnT-FGF21 Generated for this study by the Molkentin Laboratory N/A Biological samples Myocardial tissue from humans National Disease Research Interchange (NDRI) N/A Chemicals, peptides, and recombinant proteins DAPI Vectors Labs Cat#H-1500 Claycomb basal medium Sigma Cat # 51800C-500 Norepinephrine Sigma Cat#A0937 L-Glutamine Sigma Cat# TMS-002-C penicillin/streptomycin EMD Millipore Cat #TMS-AB2-C Lipofectamine 2000 Thermofischer Cat #11668019 PEI MAX MW 40K Polysciences Cat#24765-1; CAS#:49553-93-7 TRIzol lysis reagent ThermoFisher Cat#15596026 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 SYBR Select Master Mix Applied Biosystems Cat#4472903 BioRad FGF21 primer assay BioRad Cat#10025636 RIPA buffer RPI/VWR Cat#R26200–250.0 Pierce Protease and phosphotase inhibitor tablets Fisher Scientific Cat#PIA32961 PVDF membranes Fisher Scientific Cat#IPFL00010 Heparin Fisher Scientific Cat#NC2114549 Collagenase type II Worthington Biochemical Cat#LS004177 rh-FGF21 R&D Systems Cat #2539-FG-025/CF rm-FGF21 R&D Systems Cat #8409-FG-025/CF oxytocin peptide science Cat# 31-120-PS; CAS#50-56-6 (Continued on next page) e1 Cell Reports Medicine 6, 102125, June 17, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FGF21 Human ELISA Kit BioVendors RD191108200R Quantikine ELISA Mouse/Rat FGF-21 R&D SYSTEMS MF2100 Milliplex Inflammatory Cytokine Multiplex Millipore MCYTOMAG-70K-05 Deposited data Raw data files for RNA-seq Generated for this study; CCHMC Genomics Sequencing Facility NCBI Bioproject accession number: PRJNA1106030; Series GSE266368, GSE266957, GSE267618 Raw data files for Metabolomic Generated for this study; CCHMC Translational Metabolomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003743 Raw data files for Lipidomic Generated for this study; CCHMC Translational Lipidomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003851 Experimental models: Cell lines Human ventricular cardiomyocyte cell line (AC16) ATCC Cat#CRL-3568 Mouse cardiomyocyte cell line (HL-1) MiliporeSigma Cat#SCC065 AAVpro 293T cells Takara Bio Cat#632273 Experimental models: Organisms/strains HEP-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A CM-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A C57BL/6 Mice Jackson Laboratory Cat#000664 Oligonucleotides qRT-PCR primers Designed by the Drosatos Lab; obtained from Fisher Scientific Table S12 ASO-FGF21 Creative Biolabs Table S10 Recombinant DNA pAV-cTNT plasmid Obtained from Vigene Biosciences by the Molkentin Laboratory Cat#PM10013 Software and algorithms ImageJ ImageJ Software https://imagej.nih.gov/ij/ Graphpad Prism 10.2.1.395 Graphpad software https://www.graphpad.com/ TopSpin 3.6 Bruker BioSpin (NMR data collection/processing) https://www.bruker.com/en/products-andsolutions/mr/nmr-software/topspin.html Chenomx NMR Suite 8.1 Chenomx inc. (NMR data processing/ metabolites quantification) https://www.chenomx.com/ Vevo LAB 5.7.1 Fujifilm VisualSonic Vevo LAB (Echocardiography) https://www.visualsonics.com/ product/software/vevo-lab Progenesis QI Analysis Software Waters (LC-MS Data Analysis) https://www.waters.com/nextgen/us/en/ products/informatics-and-software/ mass-spectrometry-software/ progenesis-qi-software/progenesis-qi.html?
Generated:Article Title: Hepato-cardiac interorgan communication controls cardiac hypertrophy via combined endocrine-autocrine FGF21 signaling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF21 Novateinbio Cat#ASA-B0701; RRID:AB_3676235 pAMPKThr172 Cell Signaling Cat#2535; RRID:AB_331250 AMPK Cell Signaling Cat#2532; RRID:AB_330331 pERK1/2 Cell Signaling Cat#9101; RRID:AB_331646 ERK Cell Signaling Cat#4695; RRID:AB_390779 PPARα Santa Cruz Biotech Cat# Sc-398394; RRID:AB_2885073 Sirtuin 1 Cell Signaling Cat# 8469; RRID:AB_10999470 pAKT Ser473 Cell Signaling Cat# 9271; RRID:AB_329825 AKT Cell Signaling Cat# 9272; RRID:AB_329827 OXTR Proteintech Cat# 23045-1-AP; RRID:AB_2827425 β-Actin Santa Cruz Biotech Cat# SC-47778; RRID:AB_626632 Goat anti-Mouse IRDyeR 800CW LI-COR Cat#925–32210; RRID:AB_2687825 Goat anti-Rabbit IRDyeR 680RD LI-COR Cat#926–68071; RRID:AB_10956166 Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) Vector Labs Cat# FL-1021; N/A Bacterial and virus strains MyoAAV91A-cTnT-FGF21 Generated for this study by the Molkentin Laboratory N/A Biological samples Myocardial tissue from humans National Disease Research Interchange (NDRI) N/A Chemicals, peptides, and recombinant proteins DAPI Vectors Labs Cat#H-1500 Claycomb basal medium Sigma Cat # 51800C-500 Norepinephrine Sigma Cat#A0937 L-Glutamine Sigma Cat# TMS-002-C penicillin/streptomycin EMD Millipore Cat #TMS-AB2-C Lipofectamine 2000 Thermofischer Cat #11668019 PEI MAX MW 40K Polysciences Cat#24765-1; CAS#:49553-93-7 TRIzol lysis reagent ThermoFisher Cat#15596026 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 SYBR Select Master Mix Applied Biosystems Cat#4472903 BioRad FGF21 primer assay BioRad Cat#10025636 RIPA buffer RPI/VWR Cat#R26200–250.0 Pierce Protease and phosphotase inhibitor tablets Fisher Scientific Cat#PIA32961 PVDF membranes Fisher Scientific Cat#IPFL00010 Heparin Fisher Scientific Cat#NC2114549 Collagenase type II Worthington Biochemical Cat#LS004177 rh-FGF21 R&D Systems Cat #2539-FG-025/CF rm-FGF21 R&D Systems Cat #8409-FG-025/CF oxytocin peptide science Cat# 31-120-PS; CAS#50-56-6 (Continued on next page) e1 Cell Reports Medicine 6, 102125, June 17, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FGF21 Human ELISA Kit BioVendors RD191108200R Quantikine ELISA Mouse/Rat FGF-21 R&D SYSTEMS MF2100 Milliplex Inflammatory Cytokine Multiplex Millipore MCYTOMAG-70K-05 Deposited data Raw data files for RNA-seq Generated for this study; CCHMC Genomics Sequencing Facility NCBI Bioproject accession number: PRJNA1106030; Series GSE266368, GSE266957, GSE267618 Raw data files for Metabolomic Generated for this study; CCHMC Translational Metabolomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003743 Raw data files for Lipidomic Generated for this study; CCHMC Translational Lipidomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003851 Experimental models: Cell lines Human ventricular cardiomyocyte cell line (AC16) ATCC Cat#CRL-3568 Mouse cardiomyocyte cell line (HL-1) MiliporeSigma Cat#SCC065 AAVpro 293T cells Takara Bio Cat#632273 Experimental models: Organisms/strains HEP-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A CM-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A C57BL/6 Mice Jackson Laboratory Cat#000664 Oligonucleotides qRT-PCR primers Designed by the Drosatos Lab; obtained from Fisher Scientific Table S12 ASO-FGF21 Creative Biolabs Table S10 Recombinant DNA pAV-cTNT plasmid Obtained from Vigene Biosciences by the Molkentin Laboratory Cat#PM10013 Software and algorithms ImageJ ImageJ Software https://imagej.nih.gov/ij/ Graphpad Prism 10.2.1.395 Graphpad software https://www.graphpad.com/ TopSpin 3.6 Bruker BioSpin (NMR data collection/processing) https://www.bruker.com/en/products-andsolutions/mr/nmr-software/topspin.html Chenomx NMR Suite 8.1 Chenomx inc. (NMR data processing/ metabolites quantification) https://www.chenomx.com/ Vevo LAB 5.7.1 Fujifilm VisualSonic Vevo LAB (Echocardiography) https://www.visualsonics.com/ product/software/vevo-lab Progenesis QI Analysis Software Waters (LC-MS Data Analysis) https://www.waters.com/nextgen/us/en/ products/informatics-and-software/ mass-spectrometry-software/ progenesis-qi-software/progenesis-qi.html?
Recombinant:Article Title: Hepato-cardiac interorgan communication controls cardiac hypertrophy via combined endocrine-autocrine FGF21 signaling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF21 Novateinbio Cat#ASA-B0701; RRID:AB_3676235 pAMPKThr172 Cell Signaling Cat#2535; RRID:AB_331250 AMPK Cell Signaling Cat#2532; RRID:AB_330331 pERK1/2 Cell Signaling Cat#9101; RRID:AB_331646 ERK Cell Signaling Cat#4695; RRID:AB_390779 PPARα Santa Cruz Biotech Cat# Sc-398394; RRID:AB_2885073 Sirtuin 1 Cell Signaling Cat# 8469; RRID:AB_10999470 pAKT Ser473 Cell Signaling Cat# 9271; RRID:AB_329825 AKT Cell Signaling Cat# 9272; RRID:AB_329827 OXTR Proteintech Cat# 23045-1-AP; RRID:AB_2827425 β-Actin Santa Cruz Biotech Cat# SC-47778; RRID:AB_626632 Goat anti-Mouse IRDyeR 800CW LI-COR Cat#925–32210; RRID:AB_2687825 Goat anti-Rabbit IRDyeR 680RD LI-COR Cat#926–68071; RRID:AB_10956166 Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) Vector Labs Cat# FL-1021; N/A Bacterial and virus strains MyoAAV91A-cTnT-FGF21 Generated for this study by the Molkentin Laboratory N/A Biological samples Myocardial tissue from humans National Disease Research Interchange (NDRI) N/A Chemicals, peptides, and recombinant proteins DAPI Vectors Labs Cat#H-1500 Claycomb basal medium Sigma Cat # 51800C-500 Norepinephrine Sigma Cat#A0937 L-Glutamine Sigma Cat# TMS-002-C penicillin/streptomycin EMD Millipore Cat #TMS-AB2-C Lipofectamine 2000 Thermofischer Cat #11668019 PEI MAX MW 40K Polysciences Cat#24765-1; CAS#:49553-93-7 TRIzol lysis reagent ThermoFisher Cat#15596026 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 SYBR Select Master Mix Applied Biosystems Cat#4472903 BioRad FGF21 primer assay BioRad Cat#10025636 RIPA buffer RPI/VWR Cat#R26200–250.0 Pierce Protease and phosphotase inhibitor tablets Fisher Scientific Cat#PIA32961 PVDF membranes Fisher Scientific Cat#IPFL00010 Heparin Fisher Scientific Cat#NC2114549 Collagenase type II Worthington Biochemical Cat#LS004177 rh-FGF21 R&D Systems Cat #2539-FG-025/CF rm-FGF21 R&D Systems Cat #8409-FG-025/CF oxytocin peptide science Cat# 31-120-PS; CAS#50-56-6 (Continued on next page) e1 Cell Reports Medicine 6, 102125, June 17, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FGF21 Human ELISA Kit BioVendors RD191108200R Quantikine ELISA Mouse/Rat FGF-21 R&D SYSTEMS MF2100 Milliplex Inflammatory Cytokine Multiplex Millipore MCYTOMAG-70K-05 Deposited data Raw data files for RNA-seq Generated for this study; CCHMC Genomics Sequencing Facility NCBI Bioproject accession number: PRJNA1106030; Series GSE266368, GSE266957, GSE267618 Raw data files for Metabolomic Generated for this study; CCHMC Translational Metabolomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003743 Raw data files for Lipidomic Generated for this study; CCHMC Translational Lipidomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003851 Experimental models: Cell lines Human ventricular cardiomyocyte cell line (AC16) ATCC Cat#CRL-3568 Mouse cardiomyocyte cell line (HL-1) MiliporeSigma Cat#SCC065 AAVpro 293T cells Takara Bio Cat#632273 Experimental models: Organisms/strains HEP-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A CM-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A C57BL/6 Mice Jackson Laboratory Cat#000664 Oligonucleotides qRT-PCR primers Designed by the Drosatos Lab; obtained from Fisher Scientific Table S12 ASO-FGF21 Creative Biolabs Table S10 Recombinant DNA pAV-cTNT plasmid Obtained from Vigene Biosciences by the Molkentin Laboratory Cat#PM10013 Software and algorithms ImageJ ImageJ Software https://imagej.nih.gov/ij/ Graphpad Prism 10.2.1.395 Graphpad software https://www.graphpad.com/ TopSpin 3.6 Bruker BioSpin (NMR data collection/processing) https://www.bruker.com/en/products-andsolutions/mr/nmr-software/topspin.html Chenomx NMR Suite 8.1 Chenomx inc. (NMR data processing/ metabolites quantification) https://www.chenomx.com/ Vevo LAB 5.7.1 Fujifilm VisualSonic Vevo LAB (Echocardiography) https://www.visualsonics.com/ product/software/vevo-lab Progenesis QI Analysis Software Waters (LC-MS Data Analysis) https://www.waters.com/nextgen/us/en/ products/informatics-and-software/ mass-spectrometry-software/ progenesis-qi-software/progenesis-qi.html?
Lysis:Article Title: Hepato-cardiac interorgan communication controls cardiac hypertrophy via combined endocrine-autocrine FGF21 signaling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF21 Novateinbio Cat#ASA-B0701; RRID:AB_3676235 pAMPKThr172 Cell Signaling Cat#2535; RRID:AB_331250 AMPK Cell Signaling Cat#2532; RRID:AB_330331 pERK1/2 Cell Signaling Cat#9101; RRID:AB_331646 ERK Cell Signaling Cat#4695; RRID:AB_390779 PPARα Santa Cruz Biotech Cat# Sc-398394; RRID:AB_2885073 Sirtuin 1 Cell Signaling Cat# 8469; RRID:AB_10999470 pAKT Ser473 Cell Signaling Cat# 9271; RRID:AB_329825 AKT Cell Signaling Cat# 9272; RRID:AB_329827 OXTR Proteintech Cat# 23045-1-AP; RRID:AB_2827425 β-Actin Santa Cruz Biotech Cat# SC-47778; RRID:AB_626632 Goat anti-Mouse IRDyeR 800CW LI-COR Cat#925–32210; RRID:AB_2687825 Goat anti-Rabbit IRDyeR 680RD LI-COR Cat#926–68071; RRID:AB_10956166 Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) Vector Labs Cat# FL-1021; N/A Bacterial and virus strains MyoAAV91A-cTnT-FGF21 Generated for this study by the Molkentin Laboratory N/A Biological samples Myocardial tissue from humans National Disease Research Interchange (NDRI) N/A Chemicals, peptides, and recombinant proteins DAPI Vectors Labs Cat#H-1500 Claycomb basal medium Sigma Cat # 51800C-500 Norepinephrine Sigma Cat#A0937 L-Glutamine Sigma Cat# TMS-002-C penicillin/streptomycin EMD Millipore Cat #TMS-AB2-C Lipofectamine 2000 Thermofischer Cat #11668019 PEI MAX MW 40K Polysciences Cat#24765-1; CAS#:49553-93-7 TRIzol lysis reagent ThermoFisher Cat#15596026 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 SYBR Select Master Mix Applied Biosystems Cat#4472903 BioRad FGF21 primer assay BioRad Cat#10025636 RIPA buffer RPI/VWR Cat#R26200–250.0 Pierce Protease and phosphotase inhibitor tablets Fisher Scientific Cat#PIA32961 PVDF membranes Fisher Scientific Cat#IPFL00010 Heparin Fisher Scientific Cat#NC2114549 Collagenase type II Worthington Biochemical Cat#LS004177 rh-FGF21 R&D Systems Cat #2539-FG-025/CF rm-FGF21 R&D Systems Cat #8409-FG-025/CF oxytocin peptide science Cat# 31-120-PS; CAS#50-56-6 (Continued on next page) e1 Cell Reports Medicine 6, 102125, June 17, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FGF21 Human ELISA Kit BioVendors RD191108200R Quantikine ELISA Mouse/Rat FGF-21 R&D SYSTEMS MF2100 Milliplex Inflammatory Cytokine Multiplex Millipore MCYTOMAG-70K-05 Deposited data Raw data files for RNA-seq Generated for this study; CCHMC Genomics Sequencing Facility NCBI Bioproject accession number: PRJNA1106030; Series GSE266368, GSE266957, GSE267618 Raw data files for Metabolomic Generated for this study; CCHMC Translational Metabolomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003743 Raw data files for Lipidomic Generated for this study; CCHMC Translational Lipidomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003851 Experimental models: Cell lines Human ventricular cardiomyocyte cell line (AC16) ATCC Cat#CRL-3568 Mouse cardiomyocyte cell line (HL-1) MiliporeSigma Cat#SCC065 AAVpro 293T cells Takara Bio Cat#632273 Experimental models: Organisms/strains HEP-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A CM-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A C57BL/6 Mice Jackson Laboratory Cat#000664 Oligonucleotides qRT-PCR primers Designed by the Drosatos Lab; obtained from Fisher Scientific Table S12 ASO-FGF21 Creative Biolabs Table S10 Recombinant DNA pAV-cTNT plasmid Obtained from Vigene Biosciences by the Molkentin Laboratory Cat#PM10013 Software and algorithms ImageJ ImageJ Software https://imagej.nih.gov/ij/ Graphpad Prism 10.2.1.395 Graphpad software https://www.graphpad.com/ TopSpin 3.6 Bruker BioSpin (NMR data collection/processing) https://www.bruker.com/en/products-andsolutions/mr/nmr-software/topspin.html Chenomx NMR Suite 8.1 Chenomx inc. (NMR data processing/ metabolites quantification) https://www.chenomx.com/ Vevo LAB 5.7.1 Fujifilm VisualSonic Vevo LAB (Echocardiography) https://www.visualsonics.com/ product/software/vevo-lab Progenesis QI Analysis Software Waters (LC-MS Data Analysis) https://www.waters.com/nextgen/us/en/ products/informatics-and-software/ mass-spectrometry-software/ progenesis-qi-software/progenesis-qi.html?
Reverse Transcription:Article Title: Hepato-cardiac interorgan communication controls cardiac hypertrophy via combined endocrine-autocrine FGF21 signaling
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FGF21 Novateinbio Cat#ASA-B0701; RRID:AB_3676235 pAMPKThr172 Cell Signaling Cat#2535; RRID:AB_331250 AMPK Cell Signaling Cat#2532; RRID:AB_330331 pERK1/2 Cell Signaling Cat#9101; RRID:AB_331646 ERK Cell Signaling Cat#4695; RRID:AB_390779 PPARα Santa Cruz Biotech Cat# Sc-398394; RRID:AB_2885073 Sirtuin 1 Cell Signaling Cat# 8469; RRID:AB_10999470 pAKT Ser473 Cell Signaling Cat# 9271; RRID:AB_329825 AKT Cell Signaling Cat# 9272; RRID:AB_329827 OXTR Proteintech Cat# 23045-1-AP; RRID:AB_2827425 β-Actin Santa Cruz Biotech Cat# SC-47778; RRID:AB_626632 Goat anti-Mouse IRDyeR 800CW LI-COR Cat#925–32210; RRID:AB_2687825 Goat anti-Rabbit IRDyeR 680RD LI-COR Cat#926–68071; RRID:AB_10956166 Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) Vector Labs Cat# FL-1021; N/A Bacterial and virus strains MyoAAV91A-cTnT-FGF21 Generated for this study by the Molkentin Laboratory N/A Biological samples Myocardial tissue from humans National Disease Research Interchange (NDRI) N/A Chemicals, peptides, and recombinant proteins DAPI Vectors Labs Cat#H-1500 Claycomb basal medium Sigma Cat # 51800C-500 Norepinephrine Sigma Cat#A0937 L-Glutamine Sigma Cat# TMS-002-C penicillin/streptomycin EMD Millipore Cat #TMS-AB2-C Lipofectamine 2000 Thermofischer Cat #11668019 PEI MAX MW 40K Polysciences Cat#24765-1; CAS#:49553-93-7 TRIzol lysis reagent ThermoFisher Cat#15596026 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat#4368814 SYBR Select Master Mix Applied Biosystems Cat#4472903 BioRad FGF21 primer assay BioRad Cat#10025636 RIPA buffer RPI/VWR Cat#R26200–250.0 Pierce Protease and phosphotase inhibitor tablets Fisher Scientific Cat#PIA32961 PVDF membranes Fisher Scientific Cat#IPFL00010 Heparin Fisher Scientific Cat#NC2114549 Collagenase type II Worthington Biochemical Cat#LS004177 rh-FGF21 R&D Systems Cat #2539-FG-025/CF rm-FGF21 R&D Systems Cat #8409-FG-025/CF oxytocin peptide science Cat# 31-120-PS; CAS#50-56-6 (Continued on next page) e1 Cell Reports Medicine 6, 102125, June 17, 2025 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays FGF21 Human ELISA Kit BioVendors RD191108200R Quantikine ELISA Mouse/Rat FGF-21 R&D SYSTEMS MF2100 Milliplex Inflammatory Cytokine Multiplex Millipore MCYTOMAG-70K-05 Deposited data Raw data files for RNA-seq Generated for this study; CCHMC Genomics Sequencing Facility NCBI Bioproject accession number: PRJNA1106030; Series GSE266368, GSE266957, GSE267618 Raw data files for Metabolomic Generated for this study; CCHMC Translational Metabolomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003743 Raw data files for Lipidomic Generated for this study; CCHMC Translational Lipidomics Facility MetabolomicsWorkbench Project ID: PR002329 (https://doi.org/10.21228/M8KJ93) Study ID: ST003851 Experimental models: Cell lines Human ventricular cardiomyocyte cell line (AC16) ATCC Cat#CRL-3568 Mouse cardiomyocyte cell line (HL-1) MiliporeSigma Cat#SCC065 AAVpro 293T cells Takara Bio Cat#632273 Experimental models: Organisms/strains HEP-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A CM-FGF21− /− Mice Generated for this study; Drosatos Laboratory (University of Cincinnati, USA) N/A C57BL/6 Mice Jackson Laboratory Cat#000664 Oligonucleotides qRT-PCR primers Designed by the Drosatos Lab; obtained from Fisher Scientific Table S12 ASO-FGF21 Creative Biolabs Table S10 Recombinant DNA pAV-cTNT plasmid Obtained from Vigene Biosciences by the Molkentin Laboratory Cat#PM10013 Software and algorithms ImageJ ImageJ Software https://imagej.nih.gov/ij/ Graphpad Prism 10.2.1.395 Graphpad software https://www.graphpad.com/ TopSpin 3.6 Bruker BioSpin (NMR data collection/processing) https://www.bruker.com/en/products-andsolutions/mr/nmr-software/topspin.html Chenomx NMR Suite 8.1 Chenomx inc. (NMR data processing/ metabolites quantification) https://www.chenomx.com/ Vevo LAB 5.7.1 Fujifilm VisualSonic Vevo LAB (Echocardiography) https://www.visualsonics.com/ product/software/vevo-lab Progenesis QI Analysis Software Waters (LC-MS Data Analysis) https://www.waters.com/nextgen/us/en/ products/informatics-and-software/ mass-spectrometry-software/ progenesis-qi-software/progenesis-qi.html?
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